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paired end 2 × 300 bp reads (Illumina Inc)

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Illumina Inc paired end 2 × 300 bp reads
Paired End 2 × 300 Bp Reads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pair+end+reads/MiSeq+Reagent+Kit+v3/pm41865058-65-20-29
Average 99 stars, based on 11405 article reviews

paired end 2 × 300 bp reads - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Changes in Fecal Carriage of Extended-Spectrum β -Lactamase Producing Enterobacterales in Dutch Veal Calves by Clonal Spread of Klebsiella pneumoniae .
Article Snippet: DNA was loaded onto the MiSeq (Illumina) sequencer using the MiSeq Reagent kit v3 (Illumina) with pair-end reads, generating 250–300-bp read length.

Sequencing:

Article Title: Evaluating the analytical performance of direct-to-consumer gut microbiome testing services.
Article Snippet: Purified indexed samples were pooled in equal quantities and adjusted to a 4 nmol/L pool for sequencing preparation following the MiSeq Systems Denature and Dilute DNA Libraries Guide, Protocol A (Document # 15039740 v10, February 2019). .. Both 16S and WMS sequencing libraries were run using a MiSeq Reagent Kit v3 600-cycle cartridge (MS-102-3003) with pair-end reads (raw reads are be available data.nist.gov32 and BioProject ID PRJNA1364055). ..

Article Title: Experimental evaluation of the importance of colonization history in early-life gut microbiota assembly
Article Snippet: .. In brief, pair-end reads (2 300; MiSeq Reagent Kit v3) were merged and quality controlled with the merge-pairs application of the illumina-utils toolset (Eren et al., 2013) with quality check (Q30), removal of primers, removal of sequences with at least one mismatch to the primer sequence, and P parameter = 0.03 (for all other parameters default settings were used). ..

Article Title: Evaluating the analytical performance of direct-to-consumer gut microbiome testing services
Article Snippet: Purified indexed samples were pooled in equal quantities and adjusted to a 4 nmol/L pool for sequencing preparation following the MiSeq Systems Denature and Dilute DNA Libraries Guide, Protocol A (Document # 15039740 v10, February 2019). .. Both 16S and WMS sequencing libraries were run using a MiSeq Reagent Kit v3 600-cycle cartridge (MS-102-3003) with pair-end reads (raw reads are be available data.nist.gov and BioProject ID PRJNA1364055). ..

Article Title: Validation of amplicon-based next generation sequencing panel for second-tier test in newborn screening for inborn errors of metabolism
Article Snippet: .. The pooled library was then loaded to the sequencing cartridge and subject to massive parallel sequencing to generate 150 bp pair-end reads in Illumina iSeq-100 System. ..

Purification:

Article Title: Cell cycle arrest combined with CDK1 inhibition suppresses genome-wide mutations by activating alternative DNA repair genes during genome editing.
Article Snippet: A 10 kb region was amplified using PrimeSTAR GXL DNA polymerase (Takara Bio, Japan), and the products were purified using the NucleoSpin gel and polymerase chain reaction (PCR) clean-up kit (Takara Bio, Japan). .. The purified amplicons (5–10 kb) were sequenced as 151 bp pair-end reads using the iSeq 100 system (Illumina) to obtain 12,000 coverages. ..

Construct:

Article Title: Identification of essential β-oxidation genes and corresponding metabolites for oestrogen degradation by actinobacteria.
Article Snippet: Further library construction, including cDNA synthesis, adaptor ligation and enrichment, was performed according to the instruction of the TruSeq Stranded mRNA Library Prep (Illumina, San Diego, CA, USA). .. The constructed libraries were sequenced as pair-end reads (with a 301-bp read length) on the Illumina MiSeq system (Illumina, San Diego, CA, USA). ..